Format
96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate
Antibody Specificity
Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)
Species Reactivity
Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit
Assay Steps
Spin serum in Spinfilters (included) for 30 minutes
Add 50 μL Reagent 1 to plate
Add 25 μL serum, diluted Standards, diluted Controls
Add 75 μL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 μL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 μL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 μL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter