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Description

DESCRIPTION
MW= 38.8 kDa. Full length nsp15 (endoRNAse) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-346 (NCBI reference sequence YP_009725310.1).

SEQUENCE
Numeration of the nsp15 from NCBI YP_009725310.1

        10         20         30         40         50         60 
SLENVAFNVV NKGHFDGQQG EVPVSIINNT VYTKVDGVDV ELFENKTTLP VNVAFELWAK 
        70         80         90        100        110        120 
RNIKPVPEVK ILNNLGVDIA ANTVIWDYKR DAPAHISTIG VCSMTDIAKK PTETICAPLT 
       130        140        150        160        170        180 
VFFDGRVDGQ VDLFRNARNG VLITEGSVKG LQPSVGPKQA SLNGVTLIGE AVKTQFNYYK 
       190        200        210        220        230        240 
KVDGVVQQLP ETYFTQSRNL QEFKPRSQME IDFLELAMDE FIERYKLEGY AFEHIVYGDF 
       250        260        270        280        290        300 
SHSQLGGLHL LIGLAKRFKE SPFELEDFIP MDSTVKNYFI TDAQTGSSKC VCSVIDLLLD 
       310        320        330        340 
DFVEIIKSQD LSVVSKVVKV TIDYTEISFM LWCKDGHVET FYPKLQ 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 33140 M-1cm-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.