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Description

DESCRIPTION
MW= 33.3 kDa. Full length nsp16 (2′-O-ribose methyltransferase) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-298 (NCBI reference sequence YP_009725311.1).

SEQUENCE
Numeration of the nsp16 from NCBI YP_009725311.1

        10         20         30         40         50         60 
SSQAWQPGVA MPNLYKMQRM LLEKCDLQNY GDSATLPKGI MMNVAKYTQL CQYLNTLTLA 
        70         80         90        100        110        120 
VPYNMRVIHF GAGSDKGVAP GTAVLRQWLP TGTLLVDSDL NDFVSDADST LIGDCATVHT 
       130        140        150        160        170        180 
ANKWDLIISD MYDPKTKNVT KENDSKEGFF TYICGFIQQK LALGGSVAIK ITEHSWNADL 
       190        200        210        220        230        240 
YKLMGHFAWW TAFVTNVNAS SSEAFLIGCN YLGKPREQID GYVMHANYIF WRNTNPIQLS 
       250        260        270        280        290 
SYSLFDMSKF PLKLRGTAVM SLKEGQINDM ILSLLSKGRL IIRENNRVVI SSDVLVNN
 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε280 = 56630 M-1cm-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.